Open AccessAutomatic loop centring with a high‐precision goniometer head at the SLS macromolecular crystallography beamlines
Author(s) -
Pauluhn Anuschka,
Pradervand Claude,
Rossetti Daniel,
Salathe Marco,
SchulzeBriese Clemens
Publication year - 2011
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049511011848
Subject(s) - centring , goniometer , optics , backlash , hinge , magnet , materials science , computer science , mechanical engineering , physics , artificial intelligence , engineering
Automatic loop centring has been developed as part of the automation process in crystallographic data collection at the Swiss Light Source. The procedure described here consists of an optional set‐up part, in which the background images are taken, and the actual centring part. The algorithm uses boundary and centre‐of‐mass detection at two different microscope image magnifications. Micromounts can be handled as well. Centring of the loops can be achieved in 15–26 s, depending on their initial position, and as fast as manual centring. The alignment of the sample is carried out by means of a new flexural‐hinge‐based compact goniometer head. The device features an electromagnet for robotic wet mounting of samples. The circle of confusion was measured to be smaller than 1 µm (r.m.s.); its bidirectional backlash is below 2 µm.