Open AccessExperimental procedure for the characterization of radiation damage in macromolecular crystals
Author(s) -
Leal Ricardo M. F.,
Bourenkov Gleb P.,
Svensson Olof,
Spruce Darren,
Guijarro Matias,
Popov Alexander N.
Publication year - 2011
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049511002251
Subject(s) - isotropy , metric (unit) , characterization (materials science) , macromolecule , data collection , sensitivity (control systems) , crystal (programming language) , computer science , experimental data , radiation , line (geometry) , radiation damage , materials science , biological system , optics , algorithm , physics , chemistry , mathematics , statistics , engineering , biology , geometry , biochemistry , operations management , electronic engineering , programming language
A reliable and reproducible method to automatically characterize the radiation sensitivity of macromolecular crystals at the ESRF beamlines has been developed. This new approach uses the slope of the linear dependence of the overall isotropic B ‐factor with absorbed dose as the damage metric. The method has been implemented through an automated procedure using the EDNA on‐line data analysis framework and the MxCuBE data collection control interface. The outcome of the procedure can be directly used to design an optimal data collection strategy. The results of tests carried out on a number of model and real‐life crystal systems are presented.