Open AccessIn situ study of nanotemplate‐induced growth of lysozyme microcrystals by submicrometer GISAXS
Author(s) -
Pechkova Eugenia,
Nicolini Claudio
Publication year - 2011
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049510054130
Subject(s) - grazing incidence small angle scattering , lysozyme , nucleation , materials science , microbeam , crystallography , scattering , crystallization , protein crystallization , beamline , in situ , thin film , small angle x ray scattering , nanotechnology , chemical engineering , optics , chemistry , small angle neutron scattering , physics , biochemistry , beam (structure) , neutron scattering , organic chemistry , engineering
Ultrasmall lysozyme microcrystals are grown by classical hanging‐drop vapor diffusion and by its modification using a homologous protein thin‐film template displaying long‐range order. The nucleation and growth mechanisms of lysozyme microcrystals are studied at the thin lysozyme film surface using a new in situ µGISAXS (microbeam grazing‐incidence small‐angle X‐ray scattering) technique recently developed at the microfocus beamline of the ESRF in Grenoble, France. New insight on the nucleation and crystallization processes appear to emerge.