Open AccessDesign and construction of a compact end‐station at NSRRC for circular‐dichroism spectra in the vacuum‐ultraviolet region
Author(s) -
Liu SzuHeng,
Lin YiHung,
Huang LiangJen,
Luo ShiangWen,
Tsai WanLin,
Chiang SuYu,
Fung HokSum
Publication year - 2010
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049510034308
Subject(s) - beamline , synchrotron radiation , optics , circular dichroism , wavelength , circular polarization , spectral line , materials science , synchrotron , magnetic circular dichroism , vibrational circular dichroism , radiation , physics , chemistry , crystallography , beam (structure) , astronomy , microstrip
A synchrotron‐radiation‐based circular‐dichroism end‐station has been implemented at beamline BL04B at the National Synchrotron Radiation Research Center (NSRRC) in Taiwan for biological research. The design and performance of this compact end‐station for measuring circular‐dichroism spectra in the vacuum‐ultraviolet region are described. The linearly polarized light from the beamline is converted to modulated circularly polarized light with a LiF photoelastic modulator to provide a usable wavelength region of 130–330 nm. The light spot at the sample position is 5 mm × 5 mm at a slit width of 300 µm and provides a flux greater than 1 × 10 11 photons s −1 (0.1% bandwidth) −1 . A vacuum‐compatible cell made of two CaF 2 windows has a variable path length from 1.3 µm to 1 mm and a temperature range of 253–363 K. Measured CD spectra of (1 S )‐(+)‐10‐camphorsulfonic acid and proteins demonstrated the ability of this system to extend the wavelength down to 172 nm in aqueous solution and 153 nm in hexafluoro‐2‐propanol.