Open AccessStructure analysis of group I plant nucleases
Author(s) -
Dohnálek Jan,
Koval' Tomáš,
Lipovová Petra,
Podzimek Tomáš,
Matoušek Jaroslav
Publication year - 2011
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049510030700
Subject(s) - nuclease , nucleic acid , dna , rna , bifunctional , chemistry , enzyme , recombinant dna , biochemistry , crystallization , biology , gene , catalysis , organic chemistry
Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single‐stranded and double‐stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn 2+ ions was confirmed by X‐ray fluorescence. First crystallographic data were obtained.