Open AccessA decade of user operation on the macromolecular crystallography MAD beamline ID14‐4 at the ESRF
Author(s) -
McCarthy Andrew A.,
Brockhauser Sandor,
Nurizzo Didier,
Theveneau Pascal,
Mairs Trevor,
Spruce Darren,
Guijarro Matias,
Lesourd Marc,
Ravelli Raimond B. G.,
McSweeney Sean
Publication year - 2009
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049509035377
Subject(s) - beamline , undulator , upgrade , diffractometer , optics , macromolecular substances , synchrotron , computer science , nanotechnology , beam (structure) , materials science , physics , macromolecule , chemistry , scanning electron microscope , biochemistry , operating system
ID14‐4 at the ESRF is the first tunable undulator‐based macromolecular crystallography beamline that can celebrate a decade of user service. During this time ID14‐4 has not only been instrumental in the determination of the structures of biologically important molecules but has also contributed significantly to the development of various instruments, novel data collection schemes and pioneering radiation damage studies on biological samples. Here, the evolution of ID14‐4 over the last decade is presented, and some of the major improvements that were carried out in order to maintain its status as one of the most productive macromolecular crystallography beamlines are highlighted. The experimental hutch has been upgraded to accommodate a high‐precision diffractometer, a sample changer and a large CCD detector. More recently, the optical hutch has been refurbished in order to improve the X‐ray beam quality on ID14‐4 and to incorporate the most modern and robust optical elements used at other ESRF beamlines. These new optical elements will be described and their effect on beam stability discussed. These studies may be useful in the design, construction and maintenance of future X‐ray beamlines for macromolecular crystallography and indeed other applications, such as those planned for the ESRF upgrade.