Open AccessShort‐term effects of synchrotron irradiation on vasculature and tissue in healthy mouse brain
Author(s) -
Ricard Clément,
Fernández Manuel,
Gastaldo Jérôme,
Dupin Lucie,
Somveille Laurette,
Farion Régine,
Requardt Herwig,
Vial JeanClaude,
Elleaume Hélène,
Segebarth Christoph,
Van Der Sanden Boudewijn
Publication year - 2009
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049509015428
Subject(s) - irradiation , gliosis , extravasation , synchrotron , pathology , intravital microscopy , blood–brain barrier , chemistry , in vivo , biophysics , nuclear medicine , medicine , biology , central nervous system , physics , microbiology and biotechnology , nuclear physics
The purpose of this study is to measure the effects of a tomographic synchrotron irradiation on healthy mouse brain. The cerebral cortexes of healthy nude mice were irradiated with a monochromatic synchrotron beam of 79 keV at a dose of 15 Gy in accordance with a protocol of photoactivation of cisplatin previously tested in our laboratory. Forty‐eight hours, one week and one month after irradiation, the blood brain barrier (BBB) permeability was measured in the irradiated area with intravital multiphoton microscopy using fluorescent dyes with molecular weights of 4 and 70 kDa. Vascular parameters and gliosis were also assessed using quantitative immunohistochemistry. No extravasation of the fluorescent dyes was observed in the irradiated area at any measurement time (48 h, 1 week, 1 month). It appears that the BBB remains impermeable to molecules with a molecular weight of 4 kDa and above. The vascular density and vascular surface were unaffected by irradiation and no gliosis was induced. These findings suggest that a 15 Gy/79 keV synchrotron irradiation does not induce important damage on brain vasculature and tissue on the short term following irradiation.