Open AccessAre you centered? An automatic crystal‐centering method for high‐throughput macromolecular crystallography
Author(s) -
Jain Anubhav,
Stojanoff Vivian
Publication year - 2007
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049507018110
Subject(s) - throughput , crystal (programming language) , macromolecule , computer science , process (computing) , field (mathematics) , crystallography , key (lock) , optics , materials science , physics , chemistry , mathematics , telecommunications , biochemistry , computer security , pure mathematics , wireless , programming language , operating system
Crystal centering is a key step in macromolecular X‐ray crystallography experiments. A new method using image‐processing and machine‐vision techniques allows the centering of small crystals in the X‐ray beam. This method positions crystals even when the loop is initially out of the camera's field of view and adapts to the difficulty of the experiment. The process has been tested on many diverse crystals with a 93% success rate when compared with manual centering.