Open AccessX‐ray radiation‐induced damage in DNA monitored by online Raman
Author(s) -
McGeehan John E.,
Carpentier Philippe,
Royant Antoine,
Bourgeois Dominique,
Ravelli Raimond B. G.
Publication year - 2007
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049506043251
Subject(s) - raman spectroscopy , bromide , breakage , spectral line , dna , chemistry , materials science , analytical chemistry (journal) , optics , physics , inorganic chemistry , organic chemistry , astronomy , composite material , biochemistry
High‐quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non‐symmetric bending vibration for C—Br bonds in 8‐bromo‐2′‐deoxyguanosine has been unambiguously identified at 293 cm −1 in the non‐resonant Raman spectra. The breakage of this bond could be monitored by the continuous recording of Raman spectra during X‐ray exposure, and its decay versus dose could be directly correlated with the loss of the bromide definition within the electron‐density maps as determined with MX.