Open AccessRadiolysis of proteins in the solid state: an approach by EPR and product analysis
Author(s) -
Terryn Hélène,
Deridder Véronique,
Tilquin Bernard,
SicardRoselli Cécile,
HouéeLevin Chantal
Publication year - 2005
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049505003304
Subject(s) - electron paramagnetic resonance , radiolysis , radical , capillary electrophoresis , chemistry , solvent , pulsed epr , photochemistry , analytical chemistry (journal) , nuclear magnetic resonance , chromatography , organic chemistry , magnetic resonance imaging , physics , medicine , spin echo , radiology
Radio‐induced modifications in proteins have been studied using several techniques. Electron paramagnetic resonance (EPR) was used to characterize free radicals, and analysis methods (high‐performance liquid chromatography, capillary electrophoresis) were employed to visualize final degraded forms. Whereas EPR indicates that perthiyl radicals are formed, analysis does not detect any compound in which such bonds would be broken. Since EPR signals decay with time, it is concluded that rearrangements occur at subsequent steps, in which the solvent used during the analysis might play a role.