Open AccessCrystallographic data collection using a 0.22% bandwidth multilayer
Author(s) -
Englich Ulrich,
Kazimirov Alexander,
Shen Qun,
Gruner Sol M.,
Bilderback Don H.,
Hao Quan
Publication year - 2005
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049505002645
Subject(s) - monochromator , beamline , optics , synchrotron , monochromatic color , storage ring , monocrystalline silicon , diffraction , bandwidth (computing) , physics , beam (structure) , crystallography , optoelectronics , computer science , chemistry , wavelength , computer network , silicon
To bridge the gap between traditional multilayer and crystal optics a high‐resolution multilayer monochromator with a bandwidth of 0.22% has been designed and installed on a bending‐magnet beamline (F3) at the Cornell High Energy Synchrotron Source (CHESS) to provide an unfocused monochromatic X‐ray beam for protein crystallography experiments. Crystallographic data of excellent quality from a medium‐sized protein, Concanavalin A, were collected and processed using standard crystallographic programs. The data were successfully used for a structure solution and refinement. The flux from the multilayer monochromator is enhanced, relative to that from a flat Si(111) monochromator, by a factor of 5; consequently, data collection is faster and/or smaller samples may be used. At the same time, the bandwidth is narrow enough to avoid streaked spots. This experiment suggests that multilayer optics may play a valuable role in satisfying the demands of the structural biology community for rapid X‐ray data collection, particularly at under‐utilized bending‐magnet beamlines.