Open AccessIntegration of XAS and NMR techniques for the structure determination of metalloproteins. Examples from the study of copper transport proteins
Author(s) -
Banci Lucia,
Bertini Ivano,
Mangani Stefano
Publication year - 2005
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s090904950402789x
Subject(s) - metalloprotein , x ray absorption spectroscopy , structural genomics , copper , chemistry , nuclear magnetic resonance spectroscopy , metal , protein structure , crystallography , absorption spectroscopy , physics , stereochemistry , biochemistry , organic chemistry , quantum mechanics
Nuclear magnetic resonance (NMR) is a powerful technique for protein structure determination in solution. However, when dealing with metalloproteins, NMR methods are unable to directly determine the structure of the metal site and its coordination geometry. The capability of X‐ray absorption spectroscopy (XAS) to provide the structure of a metal ion bound to a protein is then perfectly suited to complement the process of the structure determination. This aspect is particularly relevant in structural genomic projects where high throughput of structural results is the main goal. The synergism of the two techniques has been exploited in the structure determination of bacterial copper transport proteins.