Open AccessA novel BioXAS technique with sub‐millisecond time resolution to track oxidation state and structural changes at biological metal centers
Author(s) -
Haumann Michael,
Müller Claudia,
Liebisch Peter,
Neisius Thomas,
Dau Holger
Publication year - 2005
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049504027803
Subject(s) - millisecond , microsecond , oxidation state , x ray absorption spectroscopy , catalysis , chemistry , temporal resolution , photochemistry , metal , reaction intermediate , manganese , reaction mechanism , combinatorial chemistry , nanotechnology , materials science , absorption spectroscopy , organic chemistry , physics , optics , astronomy
Oxidation‐state and structural changes at the metal center are crucial for the catalytic reactions of most metalloenzymes. The characterization of reaction intermediates is a prerequisite for understanding the catalytic mechanism. Frequently, intermediates are formed on the microsecond to millisecond timescale. To follow these reactions in real time represents a major challenge in structural biology. Time‐resolved BioXAS is a particularly promising tool for resolving such intermediates. A novel approach for BioXAS, termed `sampling‐XAS', is presented. First room‐temperature sampling‐XAS results have been obtained for the manganese complex of oxygenic photosynthesis. Oxidation‐state changes are monitored with a time resolution as good as 200 µs. The current prospects and limitations as well as future perspectives of time‐resolved BioXAS are discussed.