Open AccessLiquid‐chromatography‐coupled SAXS for accurate sizing of aggregating proteins
Author(s) -
Mathew Elizabeth,
Mirza Ahmed,
Menhart Nick
Publication year - 2004
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049504014086
Subject(s) - size exclusion chromatography , sizing , small angle x ray scattering , chromatography , homogeneous , scattering , set (abstract data type) , reliability (semiconductor) , data set , filtration (mathematics) , function (biology) , chemistry , materials science , analytical chemistry (journal) , biological system , computer science , physics , mathematics , artificial intelligence , optics , statistics , statistical physics , biochemistry , programming language , power (physics) , organic chemistry , evolutionary biology , biology , enzyme , quantum mechanics
Small‐angle X‐ray scattering and size‐exclusion chromatography have been combined within a unified experimental set‐up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X‐rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.