Open AccessA versatile rapid‐mixing and flow device for X‐ray absorption spectroscopy
Author(s) -
Zhang Ke,
Liu Ruifeng,
Irving Tom,
Auld David S.
Publication year - 2004
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049503028474
Subject(s) - beamline , synchrotron , absorption (acoustics) , synchrotron radiation , advanced photon source , spectrometer , spectroscopy , fluorescence spectroscopy , materials science , x ray absorption spectroscopy , absorption spectroscopy , optics , analytical chemistry (journal) , chemistry , fluorescence , beam (structure) , physics , chromatography , quantum mechanics
A low‐temperature rapid‐mixing and flow system has been designed and implemented to monitor catalysis involving metal ions by X‐ray absorption spectroscopy at the ID‐18 beamline of the Advanced Photon Source, Argonne National Laboratory. The system will allow examination of biological metallo‐intermediates at dilute metal ion concentrations by the detection of X‐ray fluorescence. The instrument can be cooled to sub‐zero temperatures, thus lengthening the life time of a reaction intermediate. A portable UV–visible spectrometer is integrated with the flow system to monitor the sample optically. The system can also be used as a continuous‐flow device to minimize radiation‐induced sample damage by reducing sample exposure to the X‐ray beam. The integration of the stop‐flow instrument with the synchrotron beamline and X‐ray fluorescence detector systems makes it unique for time‐resolved X‐ray absorption studies of dilute biological reactions. The results of the initial testing of the system are presented.