Open AccessProtein crystal growth with a two‐liquid system and stirring solution
Author(s) -
Adachi Hiroaki,
Takano Kazufumi,
Matsumura Hiroyoshi,
Inoue Tsuyoshi,
Mori Yusuke,
Sasaki Takatomo
Publication year - 2003
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049503026980
Subject(s) - protein crystallization , crystal growth , liquid crystal , materials science , crystal (programming language) , extraction (chemistry) , volume (thermodynamics) , crystallography , chemical physics , chemical engineering , chemistry , computer science , chromatography , optoelectronics , crystallization , thermodynamics , physics , organic chemistry , programming language , engineering
We developed two novel methods for growing large, high‐quality protein crystals. A two‐liquid system enables the convenient extraction of protein crystals without causing mechanical damage due to growth at the interface between two liquids. Since this system does not require limitations on solution volume, it is also suitable for the seed technique, and for growing large crystals. Another new concept is the mild stirring of the solution using the Floating And Stirring Technique (FAST) and the Micro‐stirring technique. When compared to conventional techniques, both techniques result in a reduced number of crystals, as well as the growth of large crystals.