Open AccessX‐ray induced reduction of the crystal of high‐molecular‐weight cytochrome c revealed by microspectrophotometry
Author(s) -
Sato Midori,
Shibata Naoki,
Morimoto Yukio,
Takayama Yuki,
Ozawa Kiyoshi,
Akutsu Hideo,
Higuchi Yoshiki,
Yasuoka Noritake
Publication year - 2003
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049503023860
Subject(s) - desulfovibrio vulgaris , synchrotron radiation , chemistry , irradiation , crystal (programming language) , absorption spectroscopy , synchrotron , absorption (acoustics) , crystallography , cytochrome , x ray , materials science , optics , bacteria , physics , biology , biochemistry , enzyme , genetics , nuclear physics , computer science , programming language , composite material
The crystal structures of high‐molecular‐weight cytochrome c (HMC) from Desulfovibrio vulgaris Hildenborough in the transient and reduced states have been determined at 2.8 Å resolution. An absorption spectrum measured with microspectrophotometor indicated that about 86% of the hemes were reduced after 45 min irradiation of the X‐ray beam. Further exposure for 90 min did not significantly change the spectrum. These results suggest that HMC in the crystalline state is easily reduced by illumination of the X‐ray beam from synchrotron radiation.