Open AccessSeeing the heat – preliminary studies of cryocrystallography using infrared imaging
Author(s) -
Snell Edward H.,
Judge Russell A.,
Larson Mike,
Van Der Woerd Mark J.
Publication year - 2002
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s090904950201453x
Subject(s) - annealing (glass) , crystal (programming language) , perpendicular , materials science , infrared , protein crystallization , optics , crystallization , physics , thermodynamics , computer science , geometry , mathematics , composite material , programming language
As preparation for an extensive study that aims to image the cryocooling process of macromolecular crystals, the ability to thermally image solid objects and liquids at temperatures far below 273 K is demonstrated. In the case of a large lysozyme crystal (1.0 × 0.7 × 0.2 mm), qualitative measurements show the cooling process to take about 0.6 s with the cooling taking place in a wave starting from the face of the crystal nearest to the origin of the cryostream and ending at the point furthest away from the origin. Annealing of this lysozyme crystal, cooled under good cryoprotectant conditions, shows that cold striations form perpendicular to the cooling stream. These striations become more pronounced after successive annealing. Cryocooling of a non‐cryoprotected crystal of glucose isomerase displayed an `S‐shaped' cold front wave traveling across the sample. These preliminary results are qualitative but show the power of infrared imaging as a new tool for fundamental and practical cryocrystallography studies.