Open AccessVisualization, quantification and therapeutic evaluation of angiogenic vessels in cancer by synchrotron microangiography
Author(s) -
Sekka Takafumi,
Volchikhina Svetlana A.,
Tanaka Akira,
Hasegawa Makoto,
Tanaka Yutaka,
Ohtani Yasuo,
Tajima Tomoo,
Makuuchi Hiroyasu,
Tanaka Etsuro,
Iwata Yoshiro,
Sato Shinkichi,
Hyodo Kazuyuki,
Ando Masami,
Umetani Keiji,
Kubota Misao,
Tanioka Kenkichi,
Mori Hidezo
Publication year - 2000
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049500010967
Subject(s) - microangiography , angiogenesis , cancer , synchrotron , medicine , cancer research , pathology , transplantation , physics , nuclear physics
The usefulness of a synchrotron microangiography system for depicting, quantitating and therapeutically evaluating angiogenic vessels in cancer is illustrated. In 20 mice transplanted with murine colon cancer, sequential changes in the angiogenic vessels were determined by using synchrotron microangiography, using changes in tumor volume for reference. This system allowed the depiction and quantification of angiogenic vessels in the period from one to four weeks after transplantation. The effects of antiangiogenic therapy were evaluated by using a neutralizing antibody against vascular endothelial growth factor. The neutralizing antibody partially suppressed angiogenesis and tumor growth. Synchrotron microangiography is shown to be useful for the depiction, quantification and evaluation of angiogenic vessels in cancer.