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Solution structure of detergent micelles at conditions relevant to membrane protein crystallization
Author(s) -
Littrell K.,
Urban V.,
Tiede D.,
Thiyagarajan P.
Publication year - 2000
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s002188989901314x
Subject(s) - micelle , crystallization , ionic strength , aqueous solution , small angle neutron scattering , chemistry , glycerol , peg ratio , chemical engineering , solvent , neutron scattering , ionic bonding , membrane , chromatography , organic chemistry , scattering , ion , biochemistry , physics , finance , optics , economics , engineering
In this study small angle neutron scattering was used to characterize the formation of micelles in aqueous solutions of the detergents DMG and SPC as a function of detergent concentration and ionic strength of the solvent. The effects on the micelle structure of the additives glycerol and PEG, alone as well as in combination typical for membrane protein crystallization, were also explored. This study suggests that the micelles are rod‐like in form at the concentrations studied. The size of the micelles was observed to increase with increasing ionic strength but decrease with the addition of glycerol or PEG.