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Temperature‐induced micelle to vesicle transition: kinetic effects in the DMPC/NaC system
Author(s) -
Lesieur P.,
Kiselev M.A.,
Barsukov L.I.,
Lombardo D.
Publication year - 2000
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889899012765
Subject(s) - vesicle , micelle , chemistry , dispersity , pulmonary surfactant , phospholipid , dynamic light scattering , aqueous solution , sodium cholate , liposome , chemical engineering , colloid , kinetic energy , membrane , chromatography , crystallography , analytical chemistry (journal) , organic chemistry , nanoparticle , biochemistry , physics , quantum mechanics , engineering
The formation of spherical and monodisperse small unilamellar vesicles is observed upon temperature jumps in a mixed system composed of a phospholipid (dimyristoylphosphatidylcholine) and a bile salt (sodium cholate) in an aqueous buffer. In order to enhance the X‐ray contrast of the system a mixed water/sucrose buffer is used. The spontaneous formation of unilamellar structures from mixed micelles upon temperature increase and the reverse solubilisation of membranes upon cooling is studied by means of synchrotron radiation small‐angle X‐ray scattering. The variations of the vesicle size are presented when the temperature and the surfactant / lipid ratio are modified. The kinetic conditions leading to monodisperse vesicles as well as the reversibility of the micelle to vesicle transition are investigated.