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A method to stabilize reduced and/or gas‐treated protein crystals by flash‐cooling under a controlled atmosphere
Author(s) -
Vernède Xavier,
FontecillaCamps Juan Carlos
Publication year - 1999
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889899002678
Subject(s) - glovebox , atmosphere (unit) , crystallization , flash (photography) , protein crystallization , controlled atmosphere , substrate (aquarium) , hydrogen , oxygen , materials science , redox , chemical engineering , modified atmosphere , crystallography , chemistry , thermodynamics , optics , organic chemistry , physics , metallurgy , oceanography , engineering , geology , food science , shelf life
A customized glove box for protein crystallization under a controlled atmosphere is described along with a cryogenic technique adapted to freeze protein crystals inside the glove box and a very simple device for studying gas–protein complexes in the crystalline state at cryogenic temperatures. Using these techniques different redox states of oxygen‐sensitive crystalline proteins have been stabilized and the interaction of hydrogenase with Xe, a model for the much lighter substrate molecular hydrogen, has been studied.