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Macromolecular Cryocrystallography: Cooling, Mounting, Storage and Transportation of Crystals
Author(s) -
Parkin Sean,
Hope Håkon
Publication year - 1998
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889898005305
Subject(s) - synchrotron , frost (temperature) , crystal (programming language) , nuclear engineering , simple (philosophy) , environmental science , materials science , computer science , physics , engineering , optics , composite material , philosophy , epistemology , programming language
Simple methods are presented for handling, mounting, storage and transportation of crystals at cryogenic temperatures. They are easy to learn and have a number of technical and operational advantages over currently popular methods. In particular, the temperature of the crystal throughout all manipulations is known; it is shown never to rise above that of the warmest component of the cryogenic system, typically the cold gas stream of the low‐temperature apparatus. Crystals can be mounted and inspected in the home laboratory prior to transportation to a synchrotron, giving dramatic savings in experimental time and effort. Provided appropriate care is taken, crystals remain frost free throughout any number of mount–dismount cycles.