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Applications of Polycapillary X‐ray Optics in Protein Crystallography
Author(s) -
Li PingWei,
Bi RuChang
Publication year - 1998
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889898004166
Subject(s) - diffraction , optics , crystal (programming language) , physics , x ray crystallography , resolution (logic) , x ray , radiation , materials science , crystallography , chemistry , artificial intelligence , computer science , programming language
Using polycapillary X‐ray optics, the intensity of the Cu K α radiation from a Rigaku RU200 rotating‐anode generator was increased remarkably compared with Supper double focusing mirrors. A crystal of a double mutant of trichosanthin diffracted beyond 1.9 Å with the application of the polycapillary optics and 2.0 Å resolution data with an overall of 8.4% were collected, while the same crystal only diffracted to about 2.62 Å using the radiation from double focusing mirrors. A small crystal of acidic phospholipase A2 from snake venom diffracted to 2.2 Å employing the polycapillary optics and the overall for the data collected was 8.6%. The same crystal only diffracted to about 2.7 Å using double focusing mirrors. The average intensity of reflections and the signal‐to‐noise ratio for both of the crystals increased dramatically with the application of the polycapillary X‐ray optics. This new kind of X‐ray optic has been used to collect good‐quality diffraction data for a number of protein crystals.