Premium
Simultaneous Multiwavelength Anomalous Diffraction (SMAD): an Energy‐Dispersive Synchrotron Diffraction Method for Macromolecules
Author(s) -
Lee P. L.,
Ogata C. M.
Publication year - 1995
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889895007084
Subject(s) - monochromator , diffraction , synchrotron , optics , crystal (programming language) , wavelength , x ray crystallography , materials science , synchrotron radiation , physics , crystallography , chemistry , computer science , programming language
A new synchrotron data acquisition method, simultaneous multiwavelength anomalous diffraction (SMAD), employing a variable bandwidth curved crystal monochromator (polychromator) coupled with an energy‐selecting grid plate is described. The SMAD experiment demonstrates the ability to measure reflections at six different wavelengths and their Bijvoet pairs at the same time. On one diffraction image, both the dispersive and Bijvoet information is recorded from a myoglobin crystal for multiwavelength anomalous diffraction (MAD) phasing.