Premium
Flash freezing of protein crystals: investigation of mosaic spread and diffraction limit with variation of cryoprotectant concentration
Author(s) -
Mitchell E. P.,
Garman E. F.
Publication year - 1994
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889894008629
Subject(s) - mosaicity , cryoprotectant , glycerol , diffraction , chemistry , flash (photography) , synchrotron , crystallography , analytical chemistry (journal) , chromatography , materials science , x ray crystallography , optics , physics , biochemistry , cryopreservation , biology , embryo , microbiology and biotechnology
The effect of a cryoprotectant (glycerol) upon the mosaicity of flash‐frozen T‐state glycogen phosphorylase b crystals has been investigated. Glycerol concentration in a buffered solution was varied between 0 and 70% v / v . The results show that there is a well defined optimum in glycerol concentration giving lowest mosaicity, maximum resolution and no ice formation. The results also demonstrate how flash‐freezing conditions can be systematically determined in‐house using a minimum number of crystals and before using synchrotron time.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom