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An open‐flow cryogenic cooler for single‐crystal diffraction experiments
Author(s) -
Bellamy H. D.,
Phizackerley R. P.,
Soltis S. M.,
Hope H.
Publication year - 1994
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889894006357
Subject(s) - nozzle , ice crystals , synchrotron radiation , flash (photography) , materials science , rotation (mathematics) , sample (material) , crystal (programming language) , synchrotron , flow (mathematics) , diffraction , single crystal , optics , nuclear engineering , mechanics , crystallography , computer science , chemistry , thermodynamics , physics , engineering , artificial intelligence , programming language
A simple, low‐cost and efficient open‐flow cryogenic cooling system has been constructed for crystallographic data collection from flash‐cooled protein crystals on the rotation‐camera station at the Stanford Synchrotron Radiation Laboratory. A constant temperature of less than 100 K at the crystal position can be maintained for an indefinite period of time. A small heater surrounding the delivery nozzle and another between the sample crystal and the sample support are used to prevent the formation of ice. The system includes an automatic filling capability so that the device can be run unattended for up to approximately one week.