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Volume‐specific amino acid analysis: a method for Z a determination
Author(s) -
Kwong P. D.,
Pound A.,
Hendrickson W. A.
Publication year - 1994
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889893012592
Subject(s) - volume (thermodynamics) , nucleic acid , molecule , crystal (programming language) , protein crystallization , amino acid , crystallography , chemistry , crystal structure , biological system , materials science , computer science , physics , biology , biochemistry , thermodynamics , organic chemistry , crystallization , programming language
A general method has been developed for determining the number of molecules per asymmetric unit, Z a , of a protein crystal. The method, volume‐specific amino acid analysis, relies on the direct measurement of the number of protein molecules in a crystal as normalized by the crystal volume. The method is accurate, straightforward to implement and sensitive enough for application to small (0.002 m 3 ) crystals. Although the procedures described here are specific to proteins, extension to nucleic acid crystals should be possible.