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A high‐speed data‐collection system for large‐unit‐cell crystals using an imaging plate as a detector
Author(s) -
Sato M.,
Yamamoto M.,
Imada K.,
Katsube Y.,
Tanaka N.,
Higashi T.
Publication year - 1992
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889891012955
Subject(s) - diffractometer , detector , data acquisition , crystal (programming language) , generator (circuit theory) , physics , optics , crystallography , materials science , computer science , crystal structure , chemistry , programming language , power (physics) , quantum mechanics , operating system
A high‐speed data‐collection system for large‐unit‐cell crystals is presented, using the Fuji Imaging Plate as an X‐ray detector and a rotating‐anode generator as the X‐ray source. It is an automatic data‐acquisition system that requires almost no manual intervention. The quality of data collected on the system is discussed. Merging R values ranged from 0.04 to 0.05. Compared with a four‐circle diffractometer, data reproducibility was better, isomorphous/anomalous Patterson maps were almost identical in quality and data from a small‐molecule crystal, cytidine, were of almost the same quality. Protein structures were refinable using the data measured on the system, the final crystallographic R value of the 2.2 Å 3‐isopropylmalatedehydrogenase structure being 0.185 and that of the 1.88 Å Flammulina veltipes agglutinin structure being 0.199.