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An ASAXS study of bacteriophage T7 near the K ‐absorption edges of sulfur and phosphorus
Author(s) -
Kühnholz O.
Publication year - 1991
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889891004247
Subject(s) - sulfur , chemistry , absorption (acoustics) , anomalous scattering , crystallography , scattering , bacteriophage , dna , core (optical fiber) , absorption edge , small angle x ray scattering , particle (ecology) , phosphorus , analytical chemistry (journal) , materials science , optics , physics , escherichia coli , biochemistry , chromatography , organic chemistry , biology , optoelectronics , band gap , gene , ecology
The bacteriophage T7 is a bacterial virus containing a double‐stranded DNA packed between a protein shell and core. P atoms are constituents of the backbone of DNA and S atoms of the protein. These atoms were used as native labels for in situ structure determination by anomalous small‐angle X‐ray scattering (ASAXS). The measurements have been performed in the range of the phosphorus K ‐absorption edge and the sulfur K ‐absorption edge at HASYLAB. The resonant scattering data obtained were interpreted by model calculations. A protein core in the center of the phage particle is shown directly to exist.