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Small‐angle scattering studies on clathrin‐coated vesicles
Author(s) -
Bauer R.,
Behan M.,
Hansen S.,
Jones G.,
Mortensen K.,
Særmark T.,
Øgendal L.
Publication year - 1991
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889891001279
Subject(s) - scattering , small angle neutron scattering , neutron scattering , vesicle , clathrin , biological small angle scattering , dynamic light scattering , light scattering , materials science , small angle x ray scattering , optics , chemistry , dispersity , molecular physics , nanotechnology , nanoparticle , physics , membrane , polymer chemistry , biochemistry
Structural information of clathrin‐coated vesicles has been achieved by small‐angle X‐ray, neutron and dynamic light scattering studies. A characteristic peak in the X‐ray and neutron scattering profile (in D 2 O) from intact coated vesicles is consistent with the polygonic structure of the clathrin coat. Neutron as well as dynamic light scattering gives a coated vesicle size close to 1000 Å. Dynamic light scattering detects a distribution of sizes for the coated vesicles demonstrating polydispersity of the samples. Quick freezing and slow thawing cause breakdown of the polygonic coat and production of large aggregates, as observed by dynamic light scattering and the reduction of the peak in the X‐ray scattering profile as well as an increase in the scattering intensity at the lowest angles in the neutron scattering profile.