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An automated system for micro‐batch protein crystallization and screening
Author(s) -
Chayen N. E.,
Shaw Stewart P. D.,
Maeder D. L.,
Blow D. M.
Publication year - 1990
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889890003260
Subject(s) - crystallization , stepper , software , computer science , set (abstract data type) , sample (material) , materials science , process engineering , computer hardware , nanotechnology , engineering , chromatography , chemical engineering , chemistry , operating system , programming language
An automatic sample dispenser has been constructed to aid with protein crystallization trials. This dispenser contains a bank of Hamilton syringes driven by stepper motors under computer control which is used to set up small samples (2 μl or less) for batch crystallization. Software has been written to create a series of trials which form a two‐dimensional array of crystallization conditions. A specially designed fluoropolymer multibore microtip allows the very small volumes to be mixed and dispensed with great accuracy.