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A double cell for controlling nucleation and growth of protein crystals
Author(s) -
Przybylska M.
Publication year - 1989
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889888012270
Subject(s) - nucleation , protein crystallization , crystallization , crystal growth , decoupling (probability) , drop (telecommunication) , chemical physics , crystallography , materials science , crystal (programming language) , diffusion , chemistry , chemical engineering , thermodynamics , physics , computer science , organic chemistry , telecommunications , programming language , control engineering , engineering
A simple device for protein crystallization is described that consists of two connected cells, one for the hanging‐ or sitting‐drop vapour diffusion method and the other for changing the concentration of the reservoir solution. It has been found useful for decoupling crystal nucleation from crystal growth, for improving the size and the stability of crystals, and in the preparation of heavy‐atom derivatives.