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Strategy for data collection from protein crystals using a multiwire counter area detector diffractometer
Author(s) -
Xuong N. H.,
Nielsen C.,
Hamlin R.,
Anderson D.
Publication year - 1985
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889885010433
Subject(s) - mosaicity , diffractometer , data set , set (abstract data type) , rotation (mathematics) , detector , data collection , computer science , optics , crystal (programming language) , diffraction , algorithm , physics , mathematics , computer vision , artificial intelligence , statistics , x ray crystallography , scanning electron microscope , programming language
A strategy for data collection using a diffractometer equipped with a multiwire proportional counter is described. Data are collected using an electronic rotation method similar to an earlier developed `still' method. Data are collected as a set of three to 12 or more `runs'. A data collection `run' consists of a consecutive series of hundreds of electronic rotation pictures with ω advanced by a specific amount (from 0.07 to 0.20°, depending on the mosaicity of the crystal) during each picture. Every picture in a run is taken at the same: χ and ϕ setting angles and exposure time. Information on how to extract the intensity data from these electronic rotation pictures is given elsewhere. Here, the only concern is how to choose a set of ω , χ and ϕ angles for defining a set of runs in order to get a complete set of data in a minimum time. First the diffraction geometry is discussed, then the strategy is outlined; finally, some examples are given to illustrate how the strategy is used in some typical protein data collection problems.