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The reduction of radiation damage in protein crystals by polyethylene glycol
Author(s) -
Cascio D.,
Williams R.,
McPherson A.
Publication year - 1984
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889884011316
Subject(s) - polyethylene glycol , protein crystallization , radiation damage , polyethylene , ionic strength , ionic crystal , materials science , radiation , reduction (mathematics) , ionic bonding , chemical engineering , chemistry , crystallography , composite material , optics , organic chemistry , aqueous solution , crystallization , physics , ion , engineering , geometry , mathematics
A simple technique has been developed to lessen radiation damage of protein crystals grown from low‐ionic strength solution. The technique consists of replacing the mother liquor with solutions containing 10–20% of polyethylene glycol 4000 or 20000.