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Macromolecular crystallography with synchrotron radiation: collection and processing of data from crystals with a very large unit cell
Author(s) -
Usha R.,
Johnson J. E.,
Moras D.,
Thierry J. C.,
Fourme R.,
Kahn R.
Publication year - 1984
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889884011213
Subject(s) - monochromator , collimator , synchrotron radiation , synchrotron , optics , resolution (logic) , materials science , beam (structure) , physics , wavelength , artificial intelligence , computer science
Hexagonal crystals of cowpea mosaic virus in space group P 6 1 22 (or its enantiomorph) with a = 451 and c = 1038 Å have been analyzed using the synchrotron X‐ray source at LURE. Data were collected using an X‐ray beam of size 0.18 × 0.22 mm permitting resolution of reflections along the c * direction. The beam was defined using a multiple slit system at both the monochromator and at the collimator. A helium path was used to reduce air scatter as a specimen‐to‐film distance of 175 mm was employed. Data to 4.3 Å resolution were recorded on each film. The films were processed to 6 Å resolution using the oscillation photograph processing package developed by Rossmann [ J. Appl. Cryst. (1979), 12 , 225–238] with minor modifications to the normal procedures. One film was processed to 4.3 Å resolution establishing the feasibility of intermediate resolution analysis. Statistical analysis of the processed data showed excellent correlation between symmetry‐equivalent reflections. Post refinement of oscillation angles and lattice constants was very stable and led to significantly improved scaling of partially recorded reflections.