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Scaling and phase‐difference determination in solvent contrast variation experiments
Author(s) -
Roth M.,
LewitBentley A.,
Bentley G. A.
Publication year - 1984
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889884011031
Subject(s) - contrast (vision) , amplitude , scaling , intensity (physics) , phase contrast microscopy , phase (matter) , structure factor , computational physics , optics , chemistry , materials science , physics , mathematics , crystallography , geometry , organic chemistry
In the solvent contrast variation technique, the structure factor is a linear function of contrast, and the intensity is therefore a parabolic function of contrast. A method has been developed which scales together data measured at different contrasts on different samples. Once the data are scaled, structure‐factor amplitudes or intensities for any other contrast can be obtained by inter‐ (or extra‐)polation. The magnitude of the relative phase change between contrast, an important piece of phasing information in single‐crystal studies, can be determined.