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Small‐angle scattering of chain molecules in a hydrodynamic field. The internal rigidity of double‐stranded DNA
Author(s) -
Sjöberg B.,
Osterberg R.
Publication year - 1983
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889883010523
Subject(s) - ionic strength , persistence length , macromolecule , rigidity (electromagnetism) , scattering , root mean square , gaussian , materials science , molecule , dna , chemistry , molecular physics , crystallography , analytical chemistry (journal) , optics , physics , chromatography , aqueous solution , composite material , biochemistry , computational chemistry , organic chemistry , quantum mechanics
Small‐angle X‐ray scattering data from flowing solutions of double‐stranded calf thymus DNA, in phosphate buffer of pH 6.91 and 0.2 mol dm −3 ionic strength, have been recorded over a velocity‐gradient range from zero up to a maximum value of 15300 s −1 . In comparison with small‐angle X‐ray measurements performed on stationary solutions, the method of flowing the solution through a capillary tube provides information regarding the internal flexibility of the macromolecule in solution. All the X‐ray data recorded in this work can be explained by a slightly modified bead‐spring chain model of DNA, which consists of linear segments filled with touching beads. The segments are joined together at the ends to form a long array with complete flexibility at each joint. The lengths of the segments follow a Gaussian distribution with a root‐mean‐square length equal to 100 nm. The diameter of each bead is equal to 3.0 nm.