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Time‐resolved studies on contracting muscle using small‐angle X‐ray diffraction. I. Design of data collection system
Author(s) -
Faruqi A. R.,
Huxley H. E.
Publication year - 1978
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889878013734
Subject(s) - synchronizing , detector , computer science , gaussian , diffraction , data collection , storage ring , data set , experimental data , algorithm , optics , physics , mathematics , artificial intelligence , statistics , telecommunications , beam (structure) , quantum mechanics , transmission (telecommunications)
Small‐angle X‐ray diffraction offers a unique method for the study of muscle structure at a molecular level. Structural changes in muscle during contraction can be studied with a 10 ms time resolution with the use of a powerful X‐ray generator and electronic detectors. A data‐collection system is described which is based on a linear position‐sensitive detector capable of synchronizing data from 32 different phases of a muscle contraction cycle and storing it in different subgroups of a multichannel analyzer memory. On completion of an experiment with a muscle the complete data set is transferred via a fast link to a PDP 11/10 computer for processing and storage. A promising approach to the automated analysis of equatorial data is to assume that the pattern is a sum of a polynomial background and Gaussian peaks. A χ 2 ‐minimization procedure, which optimizes the parameters used to describe the pattern, gives excellent agreement between experimental data and the proposed model. Finally a detector system is proposed which would allow collection of data at high rates expected from a camera set on a storage ring.