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Neutron small‐angle scattering of E. coli ribosomes. A contrast variation study
Author(s) -
Parfait R.,
Koch M. H. J.,
Haas J.,
Stuhrmann H. B.,
Crichton R. R.
Publication year - 1978
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889878013680
Subject(s) - ribosome , neutron , 50s , homogeneous , 30s , rna , physics , neutron scattering , ribosomal rna , scattering , protein subunit , small angle neutron scattering , small angle scattering , chemistry , optics , nuclear physics , biochemistry , statistical physics , gene
Neutron small‐angle scattering with the contrast‐variation method has established that for 50S and 70S ribosomal particles the RNA‐protein distribution is such that the RNA component is located predominantly towards the interior and the protein towards the exterior of the particle. In contrast, the 30S subunit is much more homogeneous in its RNA‐protein distribution. The shape of the 50S subunit has been determined at low resolution.