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Small‐angle X‐ray scattering study of complexes of individual components from E. coli ribosomes
Author(s) -
Österberg R.,
Sjöberg B.,
Liljas A.,
Garrett R. A.,
Littlechild J.
Publication year - 1978
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889878013679
Subject(s) - ribosome , rna , ribosomal protein , ribosomal rna , scattering , small angle x ray scattering , crystallography , small rna , biology , chemistry , biochemistry , physics , gene , optics
As part of a general X‐ray scattering study on individual ribosomal components and their specific complexes, this study deals with the proteins S1, S8, S15, S16 and S20, the S4‐binding region of 16‐S RNA, S4‐RNA, as well as the specific complexes 5‐S RNA–L18, 5‐S RNA–L18–L25 and S4–RNA–S4; the proteins were prepared under non‐denaturing conditions. In agreement with the previous X‐ray scattering studies (Österberg, Sjöberg, Liljas & Pettersson, 1976; Österberg, Sjöberg & Garrett, 1976 a ; Österberg, Sjöberg, Garrett & Littlechild 1977), involving the proteins L7/L12, L18, L25 and S4, the present data indicate that the proteins are elongated.