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Small‐angle X‐ray scattering on α‐crystallin of calf eye lens
Author(s) -
Berger J.,
Siezen R. J.
Publication year - 1978
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s002188987801362x
Subject(s) - eye lens , lens (geology) , crystallin , centrifuge , optics , scattering , materials science , generator (circuit theory) , chemistry , physics , biochemistry , nuclear physics , power (physics) , quantum mechanics
α‐Crystallin is one of the main structural proteins of the mammalian eye lens. The biochemistry of structural proteins of the mammalian eye lens has been reviewed by, for example, Harding & Dilley (1976). This work reports X‐ray scattering studies on the native α‐crystallin from calf eye lens. α‐Crystallin was prepared according to Hoenders & van Kamp (1972). It was concentrated by dissolving an ultra‐centrifuge pellet. Two different types of camera were used: a Kratky camera operated at a Philips PW 1130 generator, copper tube, and a laboratory‐constructed double‐focusing camera (Elliott generator GX 6).