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Röntgenkleinstwinkelstreuung an Erythrocyten
Author(s) -
Stasiecki P.,
Stuhrmann H. B.
Publication year - 1978
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889878012583
Subject(s) - scattering , collimated light , physics , atomic physics , range (aeronautics) , radius , normalization (sociology) , radius of gyration , chemistry , optics , materials science , nuclear magnetic resonance , laser , polymer , computer security , sociology , computer science , anthropology , composite material
The operational range of X‐ray small‐angle scattering was extended to particles and structures in the μ m‐range by the construction of a 50 m long scattering device. Erythrocytes were selected as a suitable object for study. They were found to have a radius of gyration of 2.3 ± 0.2 μ m, corresponding to a particle diameter of 6.0 ± 0.5 μ m. The volume was calculated from Perod's invariant and found to be 67 ± 10 μ m 3 . For the first time, absolute measurements could be done without weakening the primary beam, which allowed the direct determination of the mass of the particle. The mass of the erythrocyte was calculated to be 78 ± 20 pg. In all experiments a slit collimation was used. The divergence of the incident beam was smaller than the halfwidth of the scattering curve by a factor of 2.5. Therefore, the low‐angle region of the scattering profile was hardly influenced by distortions due to collimation disregarding the normalization factor.