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Thickness measurements of wet protein crystals in the electron microscope
Author(s) -
Dorset D. L.,
Parsons D. F.
Publication year - 1975
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889875009430
Subject(s) - electron microscope , environmental scanning electron microscope , orthorhombic crystal system , microscope , materials science , optics , diffraction , scanning electron microscope , electron , attenuation , interference microscopy , range (aeronautics) , analytical chemistry (journal) , crystallography , chemistry , physics , composite material , quantum mechanics , chromatography
With the stable environment afforded by a dynamically pumped wet specimen chamber, the thickness of a fully hydrated protein microcrystal may be obtained in an electron microscope from an incident‐beam attenuation measurement after disappearance of the diffraction pattern. Such measurements on orthorhombic catalase crystals have been successfully cross‐correlated with thickness measurements on the same crystals made with an interference light microscope over a range from a few hundred to a few thousand Ångströms.