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X‐ray diffuse scattering by proteins in solution. Consideration of solvent influence
Author(s) -
Fedorov B. A.,
Ptitsyn O. B.,
Voronin L. A.
Publication year - 1974
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889874009137
Subject(s) - myoglobin , globular protein , scattering , sperm whale , chemistry , solvent , x ray , crystallography , small angle x ray scattering , chemical physics , materials science , physics , optics , organic chemistry
Two methods of calculation of indicatrices of diffuse X‐ray scattering by globular proteins in a medium with a non‐zero electron density are described. The methods are used in calculation of curves of scattering by sperm whale native myoglobin in different solvents. The indicatrices obtained are close to the experimental. The nature of the so‐called `10 Å, maximum', which is observed on the scattering curves of globular proteins in dense solvents as well as in concentrated protein solutions and protein gels, is discussed.