Premium
Time‐of‐flight Bragg scattering from aligned stacks of lipid bilayers using the Liquids Reflectometer at the Spallation Neutron Source
Author(s) -
Pan Jianjun,
Heberle Frederick A.,
Carmichael Justin R.,
Ankner John F.,
Katsaras John
Publication year - 2012
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889812039945
Subject(s) - spallation neutron source , spallation , neutron reflectometry , neutron scattering , lipid bilayer , neutron , materials science , scattering , neutron diffraction , bilayer , time of flight , neutron source , small angle neutron scattering , phase (matter) , optics , diffraction , chemistry , membrane , physics , nuclear physics , biochemistry , organic chemistry
Time‐of‐flight (TOF) neutron diffraction experiments on aligned stacks of lipid bilayers using the horizontal Liquids Reflectometer at the Spallation Neutron Source are reported. Specific details are given regarding the instrumental setup, data collection and reduction, phase determination of the structure factors, and reconstruction of the one‐dimensional neutron scattering length density (NSLD) profile. The validity of using TOF measurements to determine the one‐dimensional NSLD profile is demonstrated by reproducing the results of two well known lipid bilayer structures. The method is then applied to show how an antimicrobial peptide affects membranes with and without cholesterol.