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Analysis of polycrystallinity in hen egg‐white lysozyme using a pnCCD
Author(s) -
Send Sebastian,
Abboud Ali,
Leitenberger Wolfram,
Weiss Manfred S.,
Hartmann Robert,
Strüder Lothar,
Pietsch Ullrich
Publication year - 2012
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889812015038
Subject(s) - diffraction , tetragonal crystal system , synchrotron radiation , x ray crystallography , lysozyme , crystallography , resolution (logic) , x ray , optics , synchrotron , crystal (programming language) , protein crystallization , lattice (music) , materials science , crystal structure , chemistry , physics , crystallization , biochemistry , programming language , artificial intelligence , computer science , acoustics , organic chemistry
A crystal of hen egg‐white lysozyme was analyzed by means of energy‐dispersive X‐ray Laue diffraction with white synchrotron radiation at 2.7 Å resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1° relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X‐ray screening of crystals of macromolecules or proteins prior to conventional X‐ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.