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Instrumental setup for high‐throughput small‐ and wide‐angle solution scattering at the X33 beamline of EMBL Hamburg
Author(s) -
Blanchet Clement E.,
Zozulya Alexey V.,
Kikhney Alexey G.,
Franke Daniel,
Konarev Peter V.,
Shang Weifeng,
Klaering Robbert,
Robrahn Bernd,
Hermes Christoph,
Cipriani Florent,
Svergun Dmitri I.,
Roessle Manfred
Publication year - 2012
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889812013490
Subject(s) - beamline , desy , throughput , optics , detector , sample (material) , scattering , small angle x ray scattering , small angle scattering , storage ring , physics , materials science , computer science , beam (structure) , telecommunications , wireless , thermodynamics
A setup is presented for automated high‐throughput measurements of small‐angle X‐ray scattering (SAXS) from macromolecular solutions on the bending‐magnet beamline X33 of EMBL at the storage ring DORIS‐III (DESY, Hamburg). A new multi‐cell compartment allows for rapid switching between in‐vacuum and in‐air operation, for digital camera assisted control of cell filling and for colour sample illumination. The beamline is equipped with a Pilatus 1 M‐W pixel detector for SAXS and a Pilatus 300 k‐W for wide‐angle scattering (WAXS), and results from the use of the Pilatus detectors for scattering studies are reported. The setup provides a broad resolution range from 100 to 0.36 nm without the necessity of changing the sample‐to‐detector distance. A new optimized robotic sample changer is installed, permitting rapid and reliable automated sample loading and cell cleaning with a required sample volume of 40 µl. All the devices are fully integrated into the beamline control software system, ensuring fully automated and user‐friendly operation (attended, unattended and remote) with a throughput of up to 15 measurements per hour.