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A sample holder for in‐house X‐ray powder diffraction studies of protein powders
Author(s) -
Frankær Christian Grundahl,
Harris Pernille,
Ståhl Kenny
Publication year - 2011
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889811039057
Subject(s) - powder diffraction , x ray absorption spectroscopy , diffraction , materials science , atomic absorption spectroscopy , pinhole (optics) , analytical chemistry (journal) , x ray crystallography , sample (material) , sample preparation , crystallography , absorption spectroscopy , chemistry , optics , chromatography , physics , quantum mechanics
A sample holder for handling samples of protein for in‐house X‐ray powder diffraction (XRPD) analysis has been made and tested on lysozyme. The use of an integrated pinhole reduced the background, and good signal‐to‐noise ratios were obtained from only 7 µl of sample, corresponding to approximately 2–3 mg of dry protein. The sample holder is further adaptable to X‐ray absorption spectroscopy (XAS) measurements. Both XRPD and XAS at the Zn K ‐edge were tested with hexameric Zn insulin.