Premium
Fast fluorescence techniques for crystallography beamlines
Author(s) -
Stepanov Sergey,
Hilgart Mark,
Yoder Derek W.,
Makarov Oleg,
Becker Michael,
Sanishvili Ruslan,
Ogata Craig M.,
Venugopalan Nagarajan,
Aragão David,
Caffrey Martin,
Smith Janet L.,
Fischetti Robert F.
Publication year - 2011
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889811016748
Subject(s) - fluorescence , absorption (acoustics) , optics , computer science , materials science , computer graphics (images) , physics
This paper reports on several developments of X‐ray fluorescence techniques for macromolecular crystallography recently implemented at the National Institute of General Medical Sciences and National Cancer Institute beamlines at the Advanced Photon Source. These include (i) three‐band on‐the‐fly energy scanning around absorption edges with adaptive positioning of the fine‐step band calculated from a coarse pass; (ii) on‐the‐fly X‐ray fluorescence rastering over rectangular domains for locating small and invisible crystals with a shuttle‐scanning option for increased speed; (iii) fluorescence rastering over user‐specified multi‐segmented polygons; and (iv) automatic signal optimization for reduced radiation damage of samples.